The Health Sciences Sequencing Core at UPMC Children's Hospital of Pittsburgh offers Illumina next generation sequencing services to the University of Pittsburgh research community. . The NIDDK aims to integrate, coordinate, and foster interdisciplinary cooperation between groups of established investigators that conduct high-quality research on digestive and/or liver diseases. Collene Jeter. 832-750-7259. The core was initially funded with startup funds, space and personnel from: The school of medicine Office of the Dean Academic Director: Rui Chen, Ph.D. Technical Director: Yumei Li, Ph.D. Preethi Gunaratne, Ph.D. , Director . Using both molecular and imaging analyses, we determined, at single cell and allele levels, the expres-sion of two well-characterized genes, GREB1 and MYC, whose activity is regulated by. Analyzing tumors for genetic changes can provide important information, such as whether a patient is a candidate for a specific targeted therapy, and is an important . Transcripts of over 13,000 EYFP+ cells were determined using single-cell RNA sequencing (scRNA-seq). BMC Bioinformatics 20(1):566, 2019. CPRIT investments in core facilities assures that cancer researchers have access to the most up-to-date technologies needed for cutting-edge cancer research. Alternatively, you may have mistakenly bookmarked the web login form instead of the actual web site you wanted to bookmark or used a link created by somebody else who made the same mistake. The Antibody-based Proteomics Core (ABP) (Antibody-Based Proteomics | BCM) at Baylor College of Medicine provides targeted antibody-based proteomic platforms for both validation and protein biomarker discovery, particularly for low abundance regulatory proteins.Services provided include reverse phase protein arrays (RPPA) with > 260 antibodies for total and phosphoproteins of major cell . 3Integrated Microscopy Core, Baylor College of Medicine, Houston, Texas, USA . Single-cell library synthesis was supported by the Single Cell Technology Core with funding from NIH Shared Instrument Grants S10OD018033, S10OD023469, S10OD025240, and P30EY002520 (to . Single-cell RNA sequencing uncovered remodeling of myeloid and lymphoid compartments. Baylor College of Medicine. We have the equipment and expertise to approach a wide range of structural problems at the 1 nm - 1 micron scale. "Single-cell transcriptome analysis can offer important clinical insights on individual cell subpopulations . The lab is well equipped with cutting-edge technologies including, inducible/conditional mouse models, viral-based circuit mapping and gene targeting, optogenetics and chemogenetics, in vivo and ex vivo electrophysiology, in vivo single-cell calcium imaging, in vivo EEG/EMG recording, functional profiling of novel drug targets, cognitive and . Bulk and single-cell RNA sequencing do not provide full characterization of tissue spatial diversity in cancer samples, and currently available in situ techniques (multiplex immunohistochemistry and imaging mass cytometry) allow for only limited analysis of a small number of targets. The specific goal of the postdoc fellows for these positions is to use a multi-disciplinary approach, including single-cell RNA sequencing, Patch-seq, multi-patch clamp . Send t least 20 ug with a minimum average concentration of 50 ng/L. Here, we focused on estrogen stimulation of the well-characterized GREB1 and MYC target genes that revealed large differences in cell-by-cell responses, and, more interestingly, between alleles within the same cell, both over time and hormone concentration. Director's Office. . Effective May 13, 2022, Baylor Genetics will pause its COVID testing services until further notice. Core Facility Support Awards - Twelve grants totaling $43,099,721. Primary tumors and core needle biopsies of metastatic lesions from PDAC patients were sequenced using the Chromium single cell RNA-Seq platform. Our core is equipped with three high-end electron cryomicroscopes and the associated equipment required to prepare optimal specimens. Team develops quality control for single cell imaging by Baylor College of Medicine Quality control pipeline for ER distribution under control conditions. This open line of communication provides a means to enhance the services provided by each core, ultimately benefitting the research investigators in each institution and scientific community. Developmental trajectories were generated. After post-doc training at Baylor . and a variety of other tumors. To understand how oxidative stress-producing immune cells inflict damage on the central nervous system, the researchers developed a method called Tox-seq. Baylor College of Medicine is now hiring a Pediatrics - Oncology (Associate Professor) in Houston, TX. When it comes to Baylor gear, we have what every Bears fan is looking for. The current study represents the first comprehensive approach to spatial transcriptomics of high-grade serous . The Noebels and Jiang laboratories in the Departments of Neuroscience/Neurology at Baylor College of Medicine are jointly seeking applicants for postdoc associate positions. Content. Single-cell RNA sequencing characterized tumor-infiltrating lymphocytes including Th cells and antigen-presenting B cells that were highly . Please join us on the Third Monday each month for various seminars focused on Single Cell and Spatial Analysis Technology and Tools and Resources. Baylor > Baylor Sciences Building > Core Research Centers > Other Shared Equipment. SCMarker performs cell-type-specific marker selection from single cell RNA sequencing data. The Baylor College of Medicine Institutional Animal Care and Use Committee approved all animal treatment protocols. Content. This project was supported by the Single-cell Genomics Core and by the Cytometry and Cell Sorting Core (CPRIT-RP180672, NIH P30 CA125123, and S10 RR024574) at Baylor College of Medicine. . L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, Texas. & Ph.D.) is University of Nevada, Reno Foundation Professor and Director for Single Cell Genomics & Genome Editing Core Labs at University of Nevada, Reno School of Medicine. PMID: 31628257. We thank the Houston Methodist Research Institute SEM core facility for assistance with instrumentation and Dr. Dongfang Liu and Dr. Peilin Zheng (Baylor College of Medicine) . 2A and SI Appendix, Fig. "Single-cell transcriptome analysis can offer important clinical insights on individual cell subpopulations . . The core was established by a cooperative agreement between the University of Pittsburgh Schools of Health Sciences and UPMC Children's Hospital. Core Research Centers; Center for Microscopy and Imaging. Using both molecular and imaging analyses, we determined, at single cell and allele levels, the expres-sion of two well-characterized genes, GREB1 and MYC, whose activity is regulated by. The seminar series will include both internal and external speakers and may also offer potential funding opportunities when available. Left . Arthur C. Curtis Professor of Skin Molecular Immunology and Professor Dermatology, and Taubman Medical Research Institute Scholar, at the University of Michigan. Baylor College of Medicine, Genomic & RNA Profiling Core Facility | One Baylor Plaza, Room 325E Houston, TX 77030 (713) 798-7699 . 2 Integrated Microscopy Core, Baylor College of Medicine, Houston, Texas, USA. The Baylor faculty/staff member must be present to swipe-in to receive the tickets. Among the many core facilities available at BCM are ones supporting cGMP manufacturing of cell and gene therapy products, Single Cell Genomics, Metabolomics, Analytical Biochemistry, Biostatistics, Pathology, Flow Cytometry, and the state . The colors indicate major clones with different genotypes. Send 2 T25 flasks at 80-100% confluence. As Director of University of Houston's Sequencing & Gene Editing Core and Moores Professor within the Department of Biology & Biochemistry, Preethi Gunaratne also holds an adjunct appointment with Baylor College of Medicine's Dan L. Duncan Cancer Center . Baylor University Waco, Texas 76798 1-800-229-5678. WACO, Texas (April 14, 2022) - Baylor University students from across the disciplines are off to a record-setting 2021-2022 academic year by receiving some of the most prestigious - and competitive - scholarships, fellowships and internships at the national and international levels, including a record-shattering 13 Fulbrights - and still counting - as well as Baylor's first Truman . Phone: (713) 798-6989 Email : atc-cryoemcore@bcm.edu Cytometry and Cell Sorting Core The Cytometry and Cell Sorting Core is a state-of-the-art facility offering assisted and unassisted flow cytometric services. Presented by: Johann E. Gudjonsson, MD, PhD. William T. Butler, MD, Endowed Chair for Distinguished Faculty. JEOL's automated . We are your home for all Bears athletic apparel and Baylor accessories. The Animal Models Core has been involved in the generation and/or study . (A) Random fields of view of. The research published in Nature represents the first reported single-cell . Single faculty and staff may request complimentary tickets for themselves and their dependents or one guest. Single faculty and staff may request complimentary tickets for themselves and their dependents or one guest. Thank you for visIting the Official Online Store of the Baylor Bears. Provides state-of-the-art mass spectrometry analysis of proteins and metabolites for both basic and clinical cancer research. Professor, Department of Molecular and Human Genetics Contact information ruichen@bcm.edu Education B.S., Tsinghua University, 1994 Ph.D., Baylor College of Medicine, 1999 Postdoc, Baylor College of Medicine, 2002 Research interests Our lab focuses on understanding the molecular mechanisms underlying development biology and human diseases. Ship at ambient temperature (18-25C/64-77F) in an insulated container by overnight courier. START AN ORDER GeneticsCOVID-19 Gene Results +1.800.411.4363 Payment Portal Phone: (713) 798-7565. . We present an ab initio method that . FACS Melody cell sorter; Beckman-Coulter Galios analyzer; Apoptosis and Cell Cycle assays, and other staining of cells; Alan Tucker Atucker@tulane.edu 504-988-7741 ; Histology Core Dina Guapp Dguapp@tulane.edu 504-988-1194 ; Tissue Culture . We recently developed Patch-seq, which combines whole-cell patch clamp recording with single-cell RNA-sequencing . The screen, developed by the genomic experts at Baylor Genetics in conjunction with Baylor College of Medicine, assesses fetal DNA for pathogenic and likely pathogenic variants in 30 genes. Baylor College of Medicine. In addition to housing multiple core research and service facilities, the Baylor Sciences Building is home to six academic departments and over a dozen interdisciplinary centers, programs, and institutes in the College of Arts & Sciences. Credit: Aislyn Schalck and Nicholas Navin Single-Cell RNA-seq Single-cell RNA-seq was performed at the Single Cell Genomics Core at Baylor College of Medicine. Email: singlecellgenomicscore . DNA Sequencing Core Justin Manges Jmanges@tulane.edu 504-988-7063 ; Flow Cytometry and Cell Sorting Core. Developing Single-cell RNAseq-based Genetic Screens to Identify Novel Targets for Cancer Immunotherapy (Weiyi Peng) - $250,000 . The expression of a gene should follow bi/multi-modal distribution in a mixed cell population if it is a marker of a specific cell . Tox-seq integrates single-cell RNA-sequencing technology with selective labeling of cells that produce oxidative stress and reveals which genes are "on" or "off," specifically in the . The services offered by SCGC are as follow: Single Cell Capture and 3' RNAseq / Feature Barcoding Library August 23rd, 2019 . Baylor College of Medicine, Genomic & RNA Profiling Core Facility | One Baylor Plaza, Room 325E The Advanced Technology Genomics Core (ATGC) is the primary, "one-stop" genomics core facility for researchers at MD Anderson Cancer Center. In addition, the Jagged-1/Notch signaling pathway was shown to regulate many aspects of tumorigenesis, including stem cell development, epithelial-to-mesenchymal transition, and immune cell homeostasis during minimal residual disease. A phylogenetic lineage in which single cells (circles) from a primary tumor evolved for a period of time before diverging to form a metastatic lineage. Primary tumors and core needle biopsies of metastatic lesions from PDAC patients were sequenced using the Chromium single cell RNA-Seq platform. SCOPIT: sample size calculations for single-cell sequencing experiments. 7 Targeted deletion of the gene encoding netrin-1 in hematopoietic lineage cells protected mice from developing abdominal aortic aneurysm, supporting a causative role of macrophage . An existing base of outstanding digestive and/or liver disease-related research is a prerequisite for the establishment of a Center. Core Facility Support Awards - Four grants totaling $14,821,043. We began by developing single-cell imaging and informatic workflows to query whether the single cell distribution of the estrogen receptor- . Supporting Information. Baylor Scott & White Research Institute (BSWRI) and Translational Genomics Research . cjeter@mdanderson.org. The Advanced Microscopy Core specializes in fluorescence microscopy modes such as super-resolution, confocal and dynamic microscopy and provide the instrument access, training, expertise and collaboration to researchers . The Dunn Collaborative Research Grant Program and the Optical Imaging and Vital Microscopy Core at Baylor College of . Shop our vast collection of Baylor apparel, including jerseys, t-shirts, sweatshirts, hats and more. JEM3200FSC (300kV) electron microscope with a field emission gun, an in-column energy filter, a cryo-chuck holding up to 3 grids, a goniometer tilt range (+/-70 tilts) with dual rotation cartridge, a Gatan K2 Summit direct electron detector, SerialEM for automated single particle and tomography data collection. Principal Investigator (s) Contact Information. It can encapsulate up to 48,000 cells in 10 minutes and can be used to identify rare cell types in heterogeneous populations with wide dynamic range. Both experimental and computational Professor / Director. Among the many core facilities available at BCM are ones supporting cGMP manufacturing of cell and gene therapy products, Single Cell Genomics, Metabolomics, Analytical Biochemistry, Biostatistics, Pathology, Flow Cytometry, and the state-of-the-art Microscopy.